Spanish Public Research Institution is offering licences of a patent consisting of a procedure of detection and quantification of lactic acid and bifid bacteria species from mixed cultures or fermented milk. Detection is based on identification with PCR and Denaturing Gradient Gel Electrophoresis (DGGE) has been demonstrated. This method distinguishes strains specifically. Useful for detecting viable probiotic bacteria in dairy industry.
DESCRIPTIONThe procedure obtained can detect and quantify four different species of lactic acid bacteria (S. thermophilus, L. bulgaricus, L. casei, L. acidophilus) and bifidobacterium B. lactis in mixed cultures in fermented milks specifically. The novelty of the procedure is distinguish among three different species of lactobacilli and another lactic acid bacteria as S. thermophilus and bifidobacterium as B. lactis, based on separation of PCR products in denaturing gradient gel electrophoresis PCR-DGGE system from specific strain´s DNA sequences. The main characteristic of this innovation is the use of only one reaction to discriminate and quantify lactic and bifid bacteria from fermented milk getting a fast, specific and simultaneus procedure for the reason that it differences bacteria target fragments at the same time. This procedure could be complemented with the offer technology (07 ES MAOT 0HC7) Antibiotic free selective medium to discriminate and quantify lactic and bifid bacteria from fermented milk..INNOVATIVE ASPECTSThe main characteristic of this innovation is the use of only one reaction to discriminate and quantify lactic and bifid bacteria from fermented milk getting a fast, specific and simultaneus procedure..COMPETITIVE ADVANTAGESUse of PCR- DGGE system in specific DNA fragment is the main advantage of this invention because it allows, Lactic acid bacteria and bifid bacteria species, identification and quantification in order to determine and verify probiotic aptitude of the product and its maintenance along the commercialization in a faster way, simple and with highly specificity..KEYWORDSLactic acid bacteria, quantification, bifid bacteria, identification, fermented milk, yogurt, probiotic, PCR-DGGE system..PATENT200601788 applied for 2006-07-03CONTACTEstrella Marotoemail: e.maroto@orgc.csic.esphone: +34 91 585 52 51FOR MORE CSIC TECHNOLOGY OFFERS CLICK HERE
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